Friday, January 24, 2020
Telecommunication Trends - Fiber Optics :: essays research papers
FIBER OPTICS IN OUR SCHOOLS Fiber optic refers to the medium and the technology associated with the transmission of information as light impulses along a glass or plastic wire or fiber, about the thickness of a human hair. Fiber optic wire carries much more information than conventional copper wire, and is far less subject to electromagnetic interference. A single glass fiber can carry the equivalent of 100 channels of television or 100,000 telephone calls, with even more capacity possible by encasing many fibers within one cable. Fiber optics was developed by Bell labs and Corning in the late 1960s. It does not experience signal degradation over distance as would coaxial cable. School districts are aware of the need for the upgrades to fiber optic cabling, but costs frequently preclude the upgrade. Federal Communication Commission (FCC) rulings in October of 2004, that relieved incumbent local exchange carriers from having to share fiber networks that reach within 500 feet of homes, have led to plans by BellSouth to boost fiber deployments. Concern among competitors is that their ability to compete for business voice service will be hurt. (Quesada, 2004). But while the unbundling protection for fiber-to-curb is a blow to competitive local exchange carriers, BellSouth plans to increase deployments of fiber-to-the-curb by 40 percent in 2005, a move that will help decrease the cost for local school districts. Since any installation of new wiring is labor-intensive and costly, it is little wonder that school districts lag behind the corporate world in obtaining this superior technology. School districts are rarely provided with enough state and local funds to expand current technologies to encompass such upgrades. There are numerous programs and opportunities in place, however, that will allow even less affluent school districts to keep pace. One such program is called the Universal Service Fund for Schools and Libraries, or more simply, ââ¬Å"E-rateâ⬠. E-rate was created by the Telecommunications Act of 1996, and is the discounted rate that schools and libraries pay for access to affordable telecommunications services. It was passed with the help of such politicians as Bill Clinton, Al Gore, and Newt Gingrich. The Act gave the Federal Communications Commission the power to expand universal access so that schools and libraries would be Internet wired. (Carolan & Keating, 1999). Since then, the trend to sign up for E-rate has swept the country. In 1999, Merrick and North Babylon students in Long Island, New York, discovered internet access when their schools opened, and Massapequa voters approved a multimillion-dollar bond issue that included Internet connection costs. Telecommunication Trends - Fiber Optics :: essays research papers FIBER OPTICS IN OUR SCHOOLS Fiber optic refers to the medium and the technology associated with the transmission of information as light impulses along a glass or plastic wire or fiber, about the thickness of a human hair. Fiber optic wire carries much more information than conventional copper wire, and is far less subject to electromagnetic interference. A single glass fiber can carry the equivalent of 100 channels of television or 100,000 telephone calls, with even more capacity possible by encasing many fibers within one cable. Fiber optics was developed by Bell labs and Corning in the late 1960s. It does not experience signal degradation over distance as would coaxial cable. School districts are aware of the need for the upgrades to fiber optic cabling, but costs frequently preclude the upgrade. Federal Communication Commission (FCC) rulings in October of 2004, that relieved incumbent local exchange carriers from having to share fiber networks that reach within 500 feet of homes, have led to plans by BellSouth to boost fiber deployments. Concern among competitors is that their ability to compete for business voice service will be hurt. (Quesada, 2004). But while the unbundling protection for fiber-to-curb is a blow to competitive local exchange carriers, BellSouth plans to increase deployments of fiber-to-the-curb by 40 percent in 2005, a move that will help decrease the cost for local school districts. Since any installation of new wiring is labor-intensive and costly, it is little wonder that school districts lag behind the corporate world in obtaining this superior technology. School districts are rarely provided with enough state and local funds to expand current technologies to encompass such upgrades. There are numerous programs and opportunities in place, however, that will allow even less affluent school districts to keep pace. One such program is called the Universal Service Fund for Schools and Libraries, or more simply, ââ¬Å"E-rateâ⬠. E-rate was created by the Telecommunications Act of 1996, and is the discounted rate that schools and libraries pay for access to affordable telecommunications services. It was passed with the help of such politicians as Bill Clinton, Al Gore, and Newt Gingrich. The Act gave the Federal Communications Commission the power to expand universal access so that schools and libraries would be Internet wired. (Carolan & Keating, 1999). Since then, the trend to sign up for E-rate has swept the country. In 1999, Merrick and North Babylon students in Long Island, New York, discovered internet access when their schools opened, and Massapequa voters approved a multimillion-dollar bond issue that included Internet connection costs.
Thursday, January 16, 2020
Indianapolis: Activity-Based Costing Essay
1- Yes, government should perform a cost analysis before privatization, especially if the government will remain as a participant in the bidding process. The cost analysis will provide the government with cost information for accurately pricing the bid. In case the government is not going to be a participant in the bidding process, the cost estimate will provide the government with an estimate on how much to pay for a service. For example, if the government wants to engage in a firm fixed price contract, a cost analysis will provide an accurate estimate of the cost and government can adjust the estimate for labor contractor rates and profit to price the contract. 2- For pothole filling activity direct labor and materials are relatively easy to trace directly, but it is more difficult to allocate indirect costs to the service. In order to apply ABC in Department of Transportation, reconciling with controllerââ¬â¢s records, the team correctly identified in phases 1 and 2 all the basic activities. There were 35 activities, one of them being ââ¬Å"pothole patchingâ⬠. The labor hours and direct materials assigned for this activity were easy to be traced so the direct labor and materials were precisely determined in the ââ¬Å"Pothole Filling Costâ⬠table. A few comments about the overhead costs: The model allocated costs for fixed assets and unused equipment, which is one of the strengths. The model also adjusted the current year capital purchases and added back the depreciation (the consumed portion of the purchase price). We think that was an appropriate decision in order to have a true cost for this activity. The city already had an accounting system to trace the depreciation, and that was an advantage. It is debatable the decision not to include the headquarters expenses. In fact those costs may vary up or down depending on who does the pothole filling ââ¬â municipal workers or private contractors. Decision was that these expense to ââ¬Å"remain in the cityâ⬠but they donââ¬â¢t necessarily have to. A trueà cost for the service would be to allocate them in the overhead costs. It is a bit unclear if the overhead costs captured in the ââ¬Å"Pothole Filling Costâ⬠table are strictly associated with pothole filling activity. We know that the team identified the indirect and support costs associated with the 35 primary activities. It is difficult however to allocate those costs to pothole filling activity only. For example how much would be Facility Expense slice for this service? We should assume that a reliable allocation method was utilized, since the other 34 activities should also have overhead costs traced for other potential bids. Regarding overhead costs allocation by region, a brief calculation reveals that some sort of allocation was utilized for costs, however it is not clear on what basis. Four fixed costs out of seven do follow the same allocation pattern, however that allocation does not follow the ââ¬Å"tons filledâ⬠ratio. We should also notice that the model analyzed the cost occurred during winter months, in order to prepare a bid for spring. If pothole filling is a season specific activity, the actual costs could be slightly different and the bid should be adjusted accordingly. Maybe a better idea was to apply the ABC model considering actual costs incurred during the previous spring. 3- Yes, letting the municipal employees see the ABC estimates and giving them the opportunity to reduce their costs was a good practice as it provided several benefits. Due to several past factors, the cityââ¬â¢s departments had been operating on a less than optimal level and had high overhead costs. For example the supervisor to worker ratio was too high, and the department of transportation carried excessive capital assets (vehicles). Sharing the ABC estimates and giving the opportunity to reduce costs allowed them to improve efficiency. The municipality was able to pinpoint the problem issues and fix them, such as half the supervisors were dismissed. This would allow the city to be competitive with the private sector in the bidding process. ABC estimate sharing provided an additional benefit as a buy-in from theà employees and the union. As the employees and the ABC estimators worked together to generate the estimates, they realized the data was showing the problems that they did not anticipate such has the high supervisor ratio. They recognized that ABC will be very essential for them to lower their costs and become competitive. The employees needed to be competitive with the private sector to keep their jobs. 4- We will assume that the ââ¬Å"Indirect Cost Poolâ⬠includes supervisorââ¬â¢s expenses (which should be reduced by 50%) and overhead costs. In this case the bids for Northwest and Northeast quadrants are calculated in Exhibit B. Comparing with the actual pothole filing costs per ton in January-March, we could clearly see a dramatic decrease of indirect costs and rolling stock costs. Overall the bid for both quadrants is by far more competitive than it would have been without the ABC analysis and cost reduction. 5- If the administration continues to outsource city services through competitive bidding and assuming the private sector is able to aggressively under bid the city, the administration will have to dismiss the idle workforce, selloff unused fixed assets and update the ABC estimates accordingly. As the number of outsourced contracts grows in count and size, the administration will also need to enhance its contracting and performance management capabilities.
Wednesday, January 8, 2020
Biography of Ashoka the Great, Indias Mauryan Emperor
Ashoka the Great (c. 304ââ¬â232 BCE) was the emperor of Indias Maurya Dynasty from 268 to 232 BCE and is remembered for his remarkable conversion to nonviolence and his merciful reign. In 265 BCE after witnessing the devastation of his own attack on the Kalinga region, he converted from being a brutal conqueror of a vast empire to a benevolent emperor who successfully ruled according to nonviolent principles. His edicts encouraged the protection of animals, mercy for criminals, and tolerance of other religions. Fast Facts: Ashoka the Great Known For: Ashoka was the ruler of Indias Mauryan Empire; after an epiphany, he became a promoter of Buddhist non-violence.Born: 304 BCE in Pataliputra, Mauryan EmpireParents: Bindusara and DharmaDied: 232 BCE in Pataliputra, Mauryan EmpireSpouse(s): Devi, Kaurwaki confirmed; many others allegedChildren: Mahinda, Kunala, Tivala, JalaukaNotable Quote: Dharma is good. And what is Dharma? It is having few faults and many goods deeds, mercy, charity, truthfulness, and purity. Early Life In 304 BCE, the second emperor of the Maurya Dynasty, Bindusara, welcomed a son named Ashoka Bindusara Maurya into the world. The boys mother Dharma was only a commoner. She had several older childrenââ¬âhalf-brothers of Ashokaââ¬âso Ashoka seemed unlikely to ever ascend the throne. Ashoka grew up to be a bold, troublesome, and cruel young man who was always extremely fond of hunting. According to legend, he killed a lion using only a wooden stick. His older half-brothers feared Ashokaà and convinced his father to post him as a general to distant frontiers of the Mauryan Empire. Ashoka proved to be a competent general, putting down a rebellion in the Punjabi city of Taxshila. Aware that his brothers viewed him as a rival for the throne, Ashoka went into exile for two years in the neighboring country of Kalinga. While he was there, he fell in love with and later married a commoner, a fisher-woman named Kaurwaki. Introduction to Buddhism Bindusara recalled his son to Maurya to help quell an uprising in Ujjain, the former capital of the Avanti Kingdom. Ashoka succeeded but was injured in the fighting. Buddhist monks tended to the wounded prince in secret so that his eldest brother, the heir-apparent Susima, would not learn of Ashokas injuries. At this time, Ashoka officially converted to Buddhism and began embracing its principles, though they were in direct conflict with his life as a general. He met and fell in love with a woman from Vidisha called Devi who also attended to his injuries during this period. The couple later married. When Bindusara died in 275 BCE, a two-year war for the throne erupted between Ashoka and his half-brothers. The Vedic sources vary on how many of Ashokas brothers diedââ¬âone says that he killed them all while another states that he killed several of them. In either case, Ashoka prevailed and became the third ruler of the Mauryan Empire. Imperial Rule For the first eight years of his reign, Ashoka waged near-constant war on surrounding regions. He had inherited a sizable empire, but he expanded it to include most of the Indian subcontinent, as well as the area from the current-day borders of Iran and Afghanistan in the west to Bangladesh and Burma border in the east. Only the southern tip of India and Sri Lankaà and the kingdom of Kalinga on the northeast coast of India remained out of his reach. In 265 BCE, Ashoka attacked Kalinga. Although it was the homeland of his second wife Kaurwaki and the king of Kalinga had sheltered Ashoka before his ascent to the throne, the Mauryan emperor gathered the largest invasion force in Indian history and launched his assault. Kalinga fought back bravely, but in the end it was defeated and all of its cities were sacked. Ashoka had led the invasion in person, and he went out into the capital city of Kalinga the morning after his victory to survey the damage. The ruined houses and bloodied corpses of nearly 150,000 slain civilians and soldiers sickened the emperor, and he experienced a religious epiphany. Although he had considered himself more or less a Buddhist prior to that day, the carnage at Kalinga led Ashoka to devote himself completely to Buddhism, and he vowed to practice ahimsa, or nonviolence,à from that day forward. Edicts Had Ashoka simply vowed to himself that he would live according to Buddhist principles, later ages would likely not remember his name. However, he published his intentions for the whole empire to read. Ashoka wrote out a series of edicts, explaining his policies and aspirations for the empire and urging others to follow his enlightened example. The Edicts of King Ashoka were carved onto pillars of stone 40 to 50 feet high and set up all around the edges of the Mauryan Empire as well as in the heart of Ashokas realm. Dozens of these pillars can still be found in India, Nepal, Pakistan, and Afghanistan. In his edicts, Ashoka vowed to care for his people like a father and promised neighboring people that they need not fear himââ¬âthat he would use only persuasion, not violence, to win people over. Ashoka noted that he had made available shade and fruit trees for the people as well as medical care for all people and animals. His concern for living things also appeared in a ban on live sacrifices and sport hunting as well as a request for respect for all other creatures, including servants. Ashoka urged his people to follow a vegetarian dietà and banned the practice of burning forests or agricultural wastes that might harbor wild animals. A long list of animals appeared on his protected species list, including bulls, wild ducks, squirrels, deer, porcupines, and pigeons. Ashoka also ruled with incredible accessibility. He noted that I consider it best to meet with people personally. To that end, he went on frequent tours around his empire. He also advertised that he would stop whatever he was doing if a matter of imperial business needed attention, even if he was having dinner or sleeping. In addition, Ashoka was very concerned with judicial matters. His attitude toward convicted criminals was quite merciful. He banned punishments such as torture, removing peoples eyes, and the death penalty, and he urged pardons for the elderly, those with families to support, and those who were doing charitable work. Finally, although Ashoka urged his people to practice Buddhist values, he fostered an atmosphere of respect for all religions. Within his empire, people followed not only the relatively new Buddhist faith but also Jainism, Zoroastrianism, Greek polytheism, and many other belief systems. Ashoka served as an example of tolerance for his subjects, and his religious affairs officers encouraged the practice of any religion. Death Ashoka the Great ruled as a just and merciful king from his epiphany in 265 until his death at the age of 72 in 232 BCE. His body was given a royal cremation ceremony. Legacy We do not know the names of most of Ashokas wives and children, however, his twin children by his first wife, a boy called Mahindra and a girl named Sanghamitra, were instrumental in converting Sri Lanka to Buddhism. After Ashokas death, the Mauryan Empire continued to exist for 50 years before going into a gradual decline. The last Mauryan emperor was Brhadrata, who was assassinated in 185 BCE by one of his generals, Pusyamitra Sunga. Although his family did not rule for long after he was gone, Ashokas principles and his examples lived on through the Vedas and his edicts, which can still be seen on pillars today. Sources Lahiri, Nayanjot.à Ashoka in Ancient India. Harvard University Press, 2015.Trainor, Kevin.à Buddhism: the Illustrated Guide. Duncan Baird, 2004.
Tuesday, December 31, 2019
World War I And II - 1057 Words
Two of the most agonizing periods in the 20th century, World War I and II, left a lasting impact on society economically, politically, and socially. As we have read in detailed archives on both world catastrophes, the nature in which violence shaped society after the war varied greatly. Ernst Jà ¼nger in Storm of Steel shares his heroic memoirs from fighting on the German front in World War I. Art Spiegelman tells the very personal and emotional story of his father, a survivor of World War IIââ¬â¢s Holocaust. Throughout the war, Ernst reminds us of his heroism and his withstanding sacrifice, up until he is badly injured and no longer able to fight. Ernst is proud of his achievements in the war, and shares his book with the world so that we can admire his heroism. Where as Art Spiegelmanââ¬â¢s parentââ¬â¢s, lived on after the Holocaust depressedââ¬âArtââ¬â¢s mother committed suicide 23 years after the war! After reading these two accounts, the biggest difference bet ween World War I and World War II, was how society reacted to the violence and harsh conditions enforced upon them. Civilians after World War I persevered their struggles to counteract the destruction that impeded their nationââ¬â¢s growth. On the other hand, Civilians of World War II were left in a state turmoil. With the formation of alliances fifty years prior to the start of the war, the enhancement of arsenal, and the preparation of combat tactics, World War I was a proverbial ticking-time bomb ready to detonate. Beginning in theShow MoreRelatedWorld War I and World War II700 Words à |à 3 PagesMany people know about World War I and World War II seeing that they are a couple of the worldââ¬â¢s most famous wars. Have you ever wondered how much you really know? This paper is here to inform you of all of the propaganda used to persuade people to follow Hitler, the Nazi party, and Stalin, terms that were used during this time period, and some of the very important people involved in World War I and in World War II. In the heat of World War I there were two groups, the Allies and the Central PowersRead MoreWar I And World War II919 Words à |à 4 Pageshistorians debate rather World War I and World War II were two different parts to the same war or if they are separate and distinct wars. Even though World War I and World War II were very different wars, and there were many years in between the two, the outcomes of World War I caused for World War II to happen because of unresolved issues. World War II is a continuation of World War I. World War I lasted four years and was the first total war in history. Before World War I Europe was doing well theyRead MoreThe War I And World War II1660 Words à |à 7 Pagesin thehistory of the world were World War I and World War II. World War I occurred from 1914 to 1918. World War I was caused by militarism, alliances, nationalism, imperialism and assassination (MANIA). The first four causations were more of a build-up to it. Then, once the Arch-Duke Franz Ferdinand was assassinated, the buildup was sparked. This can be compared to pouring gasoline on the ground and then lighting it on fire. World War II occurred from 1939 to 1945.World War II was caused by the discontentRead MoreWar I And World War II1517 Words à |à 7 PagesThroughout history the United States has been involved in many different wars. Their involvement has brought forth many significant events and changes that have affected the American way of life. I will briefly discuss the events and the United States involvement in World War I and World War II. 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Their pieces both give glimpses into the lives of the past and the violence in which those individualsRead MoreWorld War I And The War II1518 Words à |à 7 PagesWorld War II was a war that ended in much tragedy. There were over 60 million casualties. Throughout World War II there was how it started, the Nazi Regime, and some of the major battles. Much of the war was about the Germans bringing together a group of people called the Nazis. They were under the rule of Adolf Hitler. There were two groups fighting against each other: the Axis powers and the Allied powers. The Axis powers consisted of Japan, Italy, and Germany. The Allied powers were GreatRead MoreWar I And World War II771 Words à |à 4 Pagessought to stay out of both World War I and World War II only to be pulled in by intense happenings that inflicted chaos on the nations people. World War II would be a time in American history that would bring many fresh war tactics and developments, that helped to pull a depraved nation from the depths of a depression as well as pledge the freedom and prosperity of its opposing nations. Dangerous discoveries will be made in the world would be left to be governed by two world powers. Hoping to remainRead MoreWar I And World War II1264 Words à |à 6 Pages During any war, there will alwaAys be alliances made which stick even after the war has been dissolved. Postwar foreign policy after wars such as World War I and World War II was complicated and both had their similarities and differences from each other. World War I (WWI) strengthened our international relations with many countries, It also deteriorated some relations as well and set the stage for America becoming a great power. World War II (WWII) had some of the same effects, solidating our
Monday, December 23, 2019
The Energy Stored On Various Foods Using A Homemade...
The Energy Stored In Various Foods Using a Homemade Calorimeter Naomi Hernandez Little Rock Central High The Energy Stored in Various Foods Using a Homemade Calorimeter The purpose of this experiment is to investigate how the caloric energy in food varies in different foods and the correlation between high fat foods and calories using a homemade calorimeter. The hypothesis is that if foods with higher fat content have more energy than nonfat food, then fatty foods with more caloric energy will have higher measurements on the calorimeter, because fatty foods have calories than other foods. As the content of fat in food rises the energy stored in that food will be greater than nonfat foods. ââ¬Å"A calorie is a unit of energyâ⬠though it doesâ⬠¦show more contentâ⬠¦With an excess of calories, an individual could become overweight or obese, because the calories not used for used for basic functions are converted into fat for later usage. If the individual does not participate in physical activity, the remaining fat will not be burned off, leading to ex cess of fat (Abdallah, et al. n.d.). Too many empty calories, or calories with no nutritional value, increase the chances of obesity and other weight related issues. These calories derive form added sugar and solid fats (Weight Management, .n.d.) Solid fats refer to fats that are solid at room temperature such as: ââ¬Å"butter, milk fat, beef fat (tallow, suet), chicken fat, cream, pork fat (lard), stick margarine, shortening, hydrogenated oils, coconut oil, palm and palm kernel oilsâ⬠. Although some are named oils, it is because they derive from plants, they are considered solid fats because of the high concentration in saturated and fatty acids(Calories. n.d.). ââ¬Å"Fats are concentrated sources of caloriesâ⬠, and foods with added sugars or eaten in large portions can account to an excess in calories and lead to weight gain (How Do I, .n.d.). So foods with greater empty calories that tend to have solid fats lead excess in fat. For the purpose or this experiment food s that either are or have
Sunday, December 15, 2019
Bacteriophage and depolymerase Free Essays
Introduction Bacteriophage which is also known as bacteria eater refers to viruses that infect bacteria.Bacteriophages are known to only replicate within host cells and must attach to a specific receptor on the surface of a bacteria cell to initiate infection. The contact between the phage and the receptor is very definite which means that a phage type will only bind to a particular receptor molecule thus all bacteriophage are not alike. We will write a custom essay sample on Bacteriophage and depolymerase or any similar topic only for you Order Now Depolymerase refers to an enzyme that catalyses the breakdown of macromolecules to simpler molecules. Pseudomonas aeruginosa is commonly described as an opportunistic pathogen that causes morbidity and mortality in humans, animals and plants. Due to its exceptionally high metabolic versatility in utilizing numerous organic compounds and its adaptability to various conditions, it can be commonly found in terrestrial soil, fresh water environments and can grow in a variety of low-nutrient conditions. Bacteria and depolymerase are linked together due to the fact that the depolymerase helps the bacteria by breaking down large molecules and making it easier for the bacteria to attach to the host. Figure 1-Diagram of a bacteriophage Background information-basic information on Bacteriophages Bacteriophage are host specific viruses that targets only certain types of bacteria. They are made up of outer protein caspid enclosing a genetic material which could be SSRNA,dsRNA,ssDNA or dsNA.Bacteriophages are very common in places like soil, sewage or reservoirs infected by bacteria hosts. Itââ¬â¢s been suggested that bacteriophage might be an effective way to treat bacterial infections but studies showed that they are quickly removed from the body and hence show little clinical value therefore mostly used in the laboratory. Biofilms are made up of bacteria and other microbial cells attached directly to a solid phosphate or indirectly through associated expolysaccharides and other polymetric material. The nature of a biofilm depends on the nature of the attached microorganisms and of polymers which they secrete. The physiological conditions also greatly affect biofilm thickness and physical properties. Large numbers of phages are found in many environments as biofilms. The evidence of the ability of bacteriophage ââ¬âborne polysaccharide depolymerase enzymes to degrade bacterial polysaccharides have been proved (Adams Park 1956),Phage polysaccharide depolymerase are commonly seen in electron micrographs as spikes attached to the phage baseplate.In a liquid culture,degredation of bacterial capsular material by phage-borne polysaccharide depolymerise occurs in a defined sequence. The phage depolymerise binds to the capsular material which is the secondary receptor and degrades the polymer until it reaches the cell surface where it binds to an outer membrane receptor which is the primary receptor and infects the bacterium.Therefore,bacterial lysis will occur.This however results to a clear area in the lawn of growing cells host cells. The clearing that occurs is called a plaque and each plaque is believed to have originated from the replication events that began with one bacteriophage (virion).When the number of plaque forming units is counted, t he number of virus infectious units present in the original sample can be calculated. This procedure also permits the isolation of pure virus stains. If a plaque originated from a single virion, then all the virons in that plaque should be genetically identical. Some of the virus from the plaque can be picked and inoculated into a fresh bacterial culture inorder to establish a pure virus line (Madigan et al 2007) The purpose of this review is to isolate a bacteriophage capable of infecting a specific host bacterium. The improvement of an environmental sample ie water, for the presence of bacteriophages infecting a particular host can be carried out by inoculating water sample with the host organism and waiting for lysis to occur.Isolating of bacteriophage allows the determination of bacteriophage particles which are capable of initiating productive infections of their host bacterium in the original sample. The use of the selective enrichment in this review is to increase the probability that colonies of the desired organism would be formed. Any type of bacteria strain can be infected by bacteriophage like ecoli, pseudomonas, and salmonella. This study is based on the isolation of a bacteria strain of pseudomonas aeruginosa, not all pseudomonas strains are similar but Variability exists in the growth, curves and antibiotic resistance of different pseudomonas aeruginosa isolates. Different hypothesis have shown that isolates of p.aeruginosa could also possess some differences in their surface molecules and as a result differences in the phage receptors present on their surfaces. Mechanisms by which bacteria Infects host cells are:Adsorption,Sheath Contraction and Nucleic Acid Injection. Different methods are used for the isolation of bacteriophage from water according to different articles. Khan, N. H., Shii, Y., Kimata-Kino, N., Esaki, H., Nishino, T., Nishimura, M., Kogure, K. (2007). stated that bacteria can be isolated by different ways which include: Water sampling- In this process, selective and non selective agar culture were used for to isolate and identify P.aerruginosa and appropriate volumes of water samples are filtered using sterilized plates and Nutrient Broth agar. Identification by BD phoenix system-The phoenix system uses one identification (ID) and AST (antimicrobial susceptibility) combination panel with the ID substrates on one side and the microbial drugs on the other. Serotyping- Sereotyping for O-group specific abntigen was carried out by using P.aeruginosa kit and all isolates were tested for O-goup specific antigens. Purified Gel Electrophoresis-This was used to clarify genetic relatedness among the strains. Wuthiekanun V,Dance DAB,Wattanagon Y,SupputtamonGKOL Y,Chaowagul W and White N.J(1990) isolated pseudomonas using Ashdownââ¬â¢s medium.The use of this KEY WORDS: Bacteriophage, Depolymerase, Biofilm, Pseudomonas, REFERENCES De Groat, A (1927) The bacteriophage: method of isolation: journal of immunology.14:175-179. Khan, N. H., Shii, Y., Kimata-Kino, N., Esaki, H., Nishino, T., Nishimura, M., Kogure, K. (2007). Isolation of Pseudomonas aeruginosa from open ocean and comparison with freshwater, clinical, and animal isolates. Microbial Ecology, 53(2), 173-186. doi: 10.1007/s00248-006-9059-3 Kenezevic P, Kostanjsek, R, Obrenht D,and petrovic, O (2009) Isolation of pseudomonas aeruginosa specific phage with broad activity spectra : curr Microbiol :59:173-180 Wuthiekanun V,Dance DAB,Wattanagon Y,SupputtamonGKOL Y,Chaowagul W and White N.J(1990) The use of selective media for the isolation of pseudomonas pseudomallei in clinical practice:journal of medical microbiology Vol 33.121-126 Applied and Environmental Microbiology, April 2000, p. 1416-1422, Vol. 66, No. 4 0099-2240/00/$ Microbiology and immunology online.http://pathmicro.med.sc.edu/mayer/phage.htm http://mic.sgmjournals.org/cgi/reprint/135/10/2569?view=longpmid=2632664 http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.1998.853541.x/pdf http://www.ncbi.nlm.nih.gov/pubmed/9750288?log$=activity http://mic.sgmjournals.org/cgi/reprint/144/11/3039.pdf How to cite Bacteriophage and depolymerase, Essay examples
Friday, December 6, 2019
European Self Reflection
Question: Discuss about the European Self Reflection. Answer: It is important to engage self-reflection on one's experience over a given subject (Bruun, Lammers, Srensen, 2013). We are exposed to various issues in our communities, families, and workplaces. This personal reflection explains my experience on issues facing community development and issues that arise while working as a team in a project. Teamwork promotes creative and critical thinking skills for the members (Littlejohn, Milligan, Margaryan, 2012). I learned that the achievement of project goals by a team has pitfalls since some members are free riders. I noted that when some members of a group are free riders, the project goals and activities get sabotaged. However, with effective collaboration, the team produced reasonable results. What made me happy is how the project manager assigned duties to every member to control free riding. Some team members did not want to share their ideas, never assisted the group to solve conflicts, never listened to colleagues ideas, and let their partners do all their works. I learned that, when some members of the panel get lazy, the project goals cannot get realized. Also, I noted that it is important to share my ideas with the partners so as to make sound decisions about the project success. I was happy of how the project manager emphasized on team work. Personally, I have been working with different teams who come from various communities. In all groups, we have made numerous improvements on how we handle tasks. We have improved our technology by creating a server set up over which ideas about our projects are shared. Unlike the past, we can communicate on one platform and invite different perspectives on how to achieve project results. I learned that the success of a project is both personal and a collective act. Individual team members innovate ideas and share them collectively with the entire group. I observed that once individual ideas get respect from the team, then a collective thought is encouraged. I noted that collective knowledge is supported by virtual interactions amongst the group. In my field work internship, I learned that people from different communities have different social beliefs and norms of life. My project manager informed me that while interviewing community members from various areas on their perceptions of drug abuse, I should approach the people differently (Littlejohn, Milligan, Margaryan, 2012). I noted that the various communities have different opinions on substance abuse due to their cultural beliefs. In respect to drug usage by communities, I have seen the local leaders doing something good to address this matter. From their feedback, I realized that the local government authorities have been organizing anti-drug consumption road shows in the community to educate people on the adverse effects of its use. However, I see for myself people carelessly drinking in bars. I am glad that everyone who we interviewed in the field work gave maximum cooperation and did not frustrate us. I am so proud to be one of the reasons behind this success, and I am assured that every team member was happy about how the entire project was a success. I enjoyed working with the team and the community. References Bruun, L., Lammers, K., Srensen, G. (Eds.). (2013).European Self-reflection Between Politics and Religion: The Crisis of Europe in the 20th Century. Springer. Littlejohn, A., Milligan, C., Margaryan, A. (2012). Charting collective knowledge:Supporting self-regulated learning in the workplace.Journal of Workplace Learning,24(3), 226-238.
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